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e. coli topo iv  (Nimesh Corporation)

 
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    Nimesh Corporation e. coli topo iv
    E. Coli Topo Iv, supplied by Nimesh Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e%2E+coli+topo+iv/e++coli+topo+iv/pm33142421-667-13-26
    Average 90 stars, based on 1 article reviews
    e. coli topo iv - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Recent advancements in the medicinal chemistry of bacterial type II topoisomerase inhibitors.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.



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    MIC of EN-7 against Lab and Clinical Strains of S. aureus and E. coli

    Journal: Cell Chemical Biology

    Article Title: Discovery of a Novel DNA Gyrase-Targeting Antibiotic through the Chemical Perturbation of Streptomyces venezuelae Sporulation

    doi: 10.1016/j.chembiol.2019.06.002

    Figure Lengend Snippet: MIC of EN-7 against Lab and Clinical Strains of S. aureus and E. coli

    Article Snippet: E. coli Topo IV Decatenation Kit , Inspiralis Inc. , Cat#D4001.

    Techniques:

    In Vitro Inhibition of Topoisomerase Function by EN-7 pBR322 DNA was incubated with gyrase or topoisomerase IV and different concentrations of compound and separated by agarose gel electrophoresis. (+) is a positive control with untreated enzyme, while (−) is a no-enzyme control. Labels indicate relaxed (R), supercoiled (SC), linear (L), catenated (C), and decatenated (D) DNA. (A) Inhibition of S. aureus gyrase supercoiling by EN-7. (B) Inhibition of E. coli gyrase supercoiling by EN-7. (C) Inhibition of E. coli topoisomerase IV decatenation by EN-7. (D) Inhibition of S. aureus topoisomerase IV decatenation by EN-7. (E) Unlike ciprofloxacin (CFX), EN-7 does not stabilize a double-stranded break DNA gyrase cleavage complex with S. aureus gyrase. (F) EN-7 does not inhibit the formation of ciprofloxacin (CFX) stabilized cleavage complex. (G) EN-7 and moxifloxacin (MOX) inhibit ATP-independent S. aureus gyrase-induced DNA relaxation. (H) The S. aureus gyrase mutant GyrA S84L is less susceptible to both MOX and EN-7.

    Journal: Cell Chemical Biology

    Article Title: Discovery of a Novel DNA Gyrase-Targeting Antibiotic through the Chemical Perturbation of Streptomyces venezuelae Sporulation

    doi: 10.1016/j.chembiol.2019.06.002

    Figure Lengend Snippet: In Vitro Inhibition of Topoisomerase Function by EN-7 pBR322 DNA was incubated with gyrase or topoisomerase IV and different concentrations of compound and separated by agarose gel electrophoresis. (+) is a positive control with untreated enzyme, while (−) is a no-enzyme control. Labels indicate relaxed (R), supercoiled (SC), linear (L), catenated (C), and decatenated (D) DNA. (A) Inhibition of S. aureus gyrase supercoiling by EN-7. (B) Inhibition of E. coli gyrase supercoiling by EN-7. (C) Inhibition of E. coli topoisomerase IV decatenation by EN-7. (D) Inhibition of S. aureus topoisomerase IV decatenation by EN-7. (E) Unlike ciprofloxacin (CFX), EN-7 does not stabilize a double-stranded break DNA gyrase cleavage complex with S. aureus gyrase. (F) EN-7 does not inhibit the formation of ciprofloxacin (CFX) stabilized cleavage complex. (G) EN-7 and moxifloxacin (MOX) inhibit ATP-independent S. aureus gyrase-induced DNA relaxation. (H) The S. aureus gyrase mutant GyrA S84L is less susceptible to both MOX and EN-7.

    Article Snippet: E. coli Topo IV Decatenation Kit , Inspiralis Inc. , Cat#D4001.

    Techniques: In Vitro, Inhibition, Incubation, Agarose Gel Electrophoresis, Positive Control, Control, Mutagenesis

    Journal: Cell Chemical Biology

    Article Title: Discovery of a Novel DNA Gyrase-Targeting Antibiotic through the Chemical Perturbation of Streptomyces venezuelae Sporulation

    doi: 10.1016/j.chembiol.2019.06.002

    Figure Lengend Snippet:

    Article Snippet: E. coli Topo IV Decatenation Kit , Inspiralis Inc. , Cat#D4001.

    Techniques: Virus, Recombinant, DNA Purification, Cleavage Assay, Plasmid Preparation, Software